Coding
Part:BBa_K1315003:Design
Designed by: Dimitrios Michailidis Group: iGEM14_Dundee (2014-09-25)
The rpfG gene from Xanthomonas campestris
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1091
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 619
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 523
Design Notes
The sequence had 1 EcoRI site that was mutated out by site-directed mutagenesis.
Source
The source of this part is Xanthomonas campestris pv. campestris 8004 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.