Coding

Part:BBa_K1315003:Design

Designed by: Dimitrios Michailidis   Group: iGEM14_Dundee   (2014-09-25)


The rpfG gene from Xanthomonas campestris


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1091
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 619
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 523


Design Notes

The sequence had 1 EcoRI site that was mutated out by site-directed mutagenesis.


Source

The source of this part is Xanthomonas campestris pv. campestris 8004 genomic DNA which was provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.

References